De novo expression of glutamine synthetase during transformation of hepatic stellate cells into myofibroblast-like cells.

@article{Bode1998DeNE,
  title={De novo expression of glutamine synthetase during transformation of hepatic stellate cells into myofibroblast-like cells.},
  author={Johannes G. Bode and T Peters-Regehr and Axel M. Gressner and Dieter H{\"a}ussinger},
  journal={The Biochemical journal},
  year={1998},
  volume={335 ( Pt 3)},
  pages={697-700}
}
The expression of glutamine synthetase (GS) was studied in cultured quiescent hepatic stellate cells (HSC) and during their transformation into myofibroblast-like cells. GS mRNA was detectable in quiescent HSC (1-day culture); however, the enzyme protein was not expressed, as assessed by Western blot analysis, immunocytochemistry and the absence of detectable enzyme activity. Similar findings were obtained after 2 days of culture; in addition, the mRNA levels had dropped by about 70%, but they… CONTINUE READING