De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis

@article{Li2011DeNE,
  title={De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis},
  author={Haojian Li Li and Guoqiang Zhang and Aihua Deng and Ning Chen and Tingyi Wen},
  journal={Biotechnology Letters},
  year={2011},
  volume={33},
  pages={1575-1580}
}
Wild-type B. subtilis strain W168 was de novo engineered for inosine biosynthesis. Inactivation of deoD and purA led to 0.15 ± 0.04 and 6.44 ± 0.39 g inosine/l yields, respectively. The deoDpurA double mutant accumulated 7.6 ± 0.34 g inosine/l, with a 4.7% (w/w) conversion ratio from glucose to inosine. Comparative metabolic flux analysis revealed that the fluxes from inosine to hypoxanthine and from inosine monophosphate to adenosine monophosphate in the double mutant decreased to 14.0 and 0… CONTINUE READING

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