De novo design of an artificial bis[4Fe-4S] binding protein.

@article{Roy2013DeND,
  title={De novo design of an artificial bis[4Fe-4S] binding protein.},
  author={Anindya Roy and Iosifina Sarrou and Michael David Vaughn and Andrei V. Astashkin and Giovanna Ghirlanda},
  journal={Biochemistry},
  year={2013},
  volume={52 43},
  pages={7586-94}
}
In nature, protein subunits containing multiple iron-sulfur clusters often mediate the delivery of reducing equivalents from metabolic pathways to the active site of redox proteins. The de novo design of redox active proteins should include the engineering of a conduit for the delivery of electrons to and from the active site, in which multiple redox active centers are arranged in a controlled manner. Here, we describe a designed three-helix protein, DSD-bis[4Fe-4S], that coordinates two iron… CONTINUE READING