De novo assembly and characterization of spotted seal Phoca largha transcriptome using Illumina paired-end sequencing.

@article{Gao2013DeNA,
  title={De novo assembly and characterization of spotted seal Phoca largha transcriptome using Illumina paired-end sequencing.},
  author={Xianggang Gao and Jiabo Han and Zhichuang Lu and Yunfeng Li and Chong-bo He},
  journal={Comparative biochemistry and physiology. Part D, Genomics & proteomics},
  year={2013},
  volume={8 2},
  pages={103-10}
}
Spotted seal (Phoca largha) is categorized as a critically endangered species in China. The aim of this study was to investigate spotted seal transcriptome by the approach of Illumina paired-end sequencing technology. We obtained a total of 52,146,394 reads for the mixed tissues of liver and spleen from the spotted seal. The de novo assemblies yielded 354,014 contigs and 178,466 unigenes. In the transcriptome, 193 unigenes were assigned to defense mechanisms. Three unigenes encoded MHC class I… CONTINUE READING