De novo DNA synthesis using polymerase-nucleotide conjugates

@article{Palluk2018DeND,
  title={De novo DNA synthesis using polymerase-nucleotide conjugates},
  author={Sebastian Palluk and Daniel H Arlow and Tristan de Rond and Sebastian Barthel and Justine S Kang and Rathin Bector and Hratch M. Baghdassarian and Alisa N Truong and Peter W Kim and Anup Kumar Singh and Nathan J. Hillson and Jay D. Keasling},
  journal={Nature Biotechnology},
  year={2018},
  volume={36},
  pages={645-650}
}
Oligonucleotides are almost exclusively synthesized using the nucleoside phosphoramidite method, even though it is limited to the direct synthesis of ∼200 mers and produces hazardous waste. Here, we describe an oligonucleotide synthesis strategy that uses the template-independent polymerase terminal deoxynucleotidyl transferase (TdT). Each TdT molecule is conjugated to a single deoxyribonucleoside triphosphate (dNTP) molecule that it can incorporate into a primer. After incorporation of the… 
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