De novo Assembly of a 40 Mb Eukaryotic Genome from Short Sequence Reads: Sordaria macrospora, a Model Organism for Fungal Morphogenesis

@inproceedings{Nowrousian2010DeNA,
  title={De novo Assembly of a 40 Mb Eukaryotic Genome from Short Sequence Reads: Sordaria macrospora, a Model Organism for Fungal Morphogenesis},
  author={Minou Nowrousian and Jason E. Stajich and Meiling Chu and Ines Engh and Eric Espagne and Karen J Halliday and Jens Kamerewerd and Frank Kempken and Birgit Knab and Hsiao-Che Kuo and Heinz D. Osiewacz and Stefanie P{\"o}ggeler and Nick D Read and Stephan Seiler and Kristina Smith and D. Zickler and Ulrich K{\"u}ck and Michael Freitag},
  booktitle={PLoS genetics},
  year={2010}
}
Filamentous fungi are of great importance in ecology, agriculture, medicine, and biotechnology. Thus, it is not surprising that genomes for more than 100 filamentous fungi have been sequenced, most of them by Sanger sequencing. While next-generation sequencing techniques have revolutionized genome resequencing, e.g. for strain comparisons, genetic mapping, or transcriptome and ChIP analyses, de novo assembly of eukaryotic genomes still presents significant hurdles, because of their large size… CONTINUE READING