De Novo Sequencing and Global Transcriptome Analysis of Nannochloropsis sp. (Eustigmatophyceae) Following Nitrogen Starvation

@article{Liang2012DeNS,
  title={De Novo Sequencing and Global Transcriptome Analysis of Nannochloropsis sp. (Eustigmatophyceae) Following Nitrogen Starvation},
  author={Chengwei Liang and Shaona Cao and Xiaowen Zhang and Baohua Zhu and Zhongliang Su and Dong-po Xu and Xiangyu Guang and Naihao Ye},
  journal={BioEnergy Research},
  year={2012},
  volume={6},
  pages={494-505}
}
Nannochloropsis sp. is an economically and nutritionally important microalga. Recently it has been demonstrated that Nannochloropsis sp. has significant potential for biofuel production. To determine the mechanisms of lipid formation and accumulation during nitrogen starvation, a transcriptomic study was performed to compare gene expression during growth with and without nitrogen. Digital expression analysis identified 1,855 differentially expressed genes between cells grown under nitrogen… 
Chromosome scale genome assembly and transcriptome profiling of Nannochloropsis gaditana in nitrogen depletion.
TLDR
A high-quality genomic assembly of Nannochloropsis gaditana, consisting of large contigs, up to 500 kbp long, and scaffolds that in most cases span the entire length of the chromosomes is reported, revealing traits relevant for the genetic improvement of this organism.
Transcriptomic and lipidomic analysis of an EPA-containing Nannochloropsis sp. PJ12 in response to nitrogen deprivation
TLDR
Comparison of transcriptomic and lipidomic analyses of cells under ND and NR conditions indicated that TAG accumulation is attributed to the modification of membrane lipids derived primarily from “prokaryotic” pathway and secondarily from "eukaryotic" pathway based on the 16:X or 18:X fatty acid at the sn2 position of the glycerol backbone.
The Combination of RNA and Protein Profiling Reveals the Response to Nitrogen Depletion in Thalassiosira pseudonana
TLDR
The RNA-Seq profile results showed that Thalassiosira pseudonana responded to N deprivation through increases in glycolysis, the TCA cycle and N metabolism as well as down-regulation in the Calvin cycle, gluconeogenesis, pentose phosphate, oxidative phosphorylation and lipid synthesis.
Comparative proteomics reveals proteins impacted by nitrogen deprivation in wild-type and high lipid-accumulating mutant strains of Tisochrysis lutea.
TLDR
The first comparative proteomic analysis on Tisochrysis lutea, a non-model marine microalga of interest for aquaculture and lipids production, confirms the impact of nitrogen starvation on overall metabolism and provides new perspectives to study the lipid over-accumulation in the prymnesiophyte haptophyte T. luteA.
RNA-Seq and transcriptome analysis of nitrogen-deprivation responsive genes in Dunaliella salina TG strain
TLDR
The transcriptome data may serve as a reference for further analysis of gene expression and functional genomics studies, and will facilitate the study of carotenoid biosynthesis and nitrogen metabolism in chlorophytes at the molecular level.
De novo transcriptome sequencing of Monodopsis subterranea CCALA 830 and identification of genes involved in the biosynthesis of eicosapentanoic acid and triacylglycerol
Monodopsis subterranea, a unicellular yellow-green freshwater microalga, is widely known for its ability to produce high amount of therapeutically beneficial ω-3 polyunsaturated fatty acid
Physiological and transcriptome analysis elucidates the metabolic mechanism of versatile Porphyridium purpureum under nitrogen deprivation for exopolysaccharides accumulation
Porphyridium purpureum is a mesophilic, unicellular red alga rich in phycoerythrin, sulfate polysaccharides, and polyunsaturated fatty acids. Nitrogen deficiency inhibited the growth of P. purpureum
Enhancement of biomass and lipid productivity by overexpression of a bZIP transcription factor in Nannochloropsis salina
TLDR
It is demonstrated that NsbZIP1 could improve both growth and lipid production, and TF engineering can serve as an excellent genetic engineering tool for production of biofuels and biomaterials in microalgae.
Metabolic Remodeling of Membrane Glycerolipids in the Microalga Nannochloropsis oceanica under Nitrogen Deprivation
TLDR
A conceptual eicosapentaenoic acid (EPA) biosynthesis network is proposed based on the lipidomic and transcriptomic data, which underlined import of EPA from cytosolic glycerolipids to the plastid for synthesize EPA-containing chloroplast membrane lipids.
Data-Independent-Acquisition-Based Proteomic Approach towards Understanding the Acclimation Strategy of Oleaginous Microalga Microchloropsis gaditana CCMP526 in Hypersaline Conditions
TLDR
Temporal proteome analysis of salinity-induced M. gaditana revealed vital SRPs which could be used for engineering salinity resilient microalgal strains for improved productivity in hypersaline culture conditions.
...
1
2
3
4
...

References

SHOWING 1-10 OF 72 REFERENCES
Changes in Transcript Abundance in Chlamydomonas reinhardtii following Nitrogen Deprivation Predict Diversion of Metabolism1[W][OA]
TLDR
Comparative global analysis of transcripts under induced and noninduced conditions was applied as a first approach to studying molecular changes that promote or accompany triacylglycerol accumulation in cells encountering a new nutrient environment, and conclusions on metabolism based on transcriptional analysis are indirect.
Potential role of multiple carbon fixation pathways during lipid accumulation in Phaeodactylum tricornutum
BackgroundPhaeodactylum tricornutum is a unicellular diatom in the class Bacillariophyceae. The full genome has been sequenced (<30 Mb), and approximately 20 to 30% triacylglyceride (TAG)
Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana
TLDR
It is shown that N. gaditana has highly favourable lipid yields, and is a promising production organism, and the availability of a genome sequence and transformation methods will facilitate investigations into N. Gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga.
The effect of light, salinity, and nitrogen availability on lipid production by Nannochloropsis sp.
TLDR
Combined higher salinity and light intensity was detrimental to lipid productivity under nitrogen starvation; biomass TFA content, and lipid productivity amounted for only 33% of DW and ca.
Photosynthetic carbon partitioning and lipid production in the oleaginous microalga Pseudochlorococcum sp. (Chlorophyceae) under nitrogen-limited conditions.
TLDR
Results suggest that Pseudochlorococcum used starch as a primary carbon and energy storage product and cells shift the carbon partitioning into neutral lipid as a secondary storage product.
Rapid estimation of lipid content in an Antarctic ice alga (Chlamydomonas sp.) using the lipophilic fluorescent dye BODIPY505/515
TLDR
It is demonstrated that nitrogen deficiency and UV-B radiation could significantly promote the accumulation of lipid content per cell in Chlamydomonas ICE-L, a promising alternative species for production of microalgal oil for the production of renewable biodiesel in the future.
Characterization of the LhcSR Gene Under Light and Temperature Stress in the Green Alga Ulva linza
TLDR
The data suggest that the LhcSR gene is involved in photoprotection in U. linza, and the results suggest a stronger link to LT.
COORDINATED CAROTENOID AND LIPID SYNTHESES INDUCED IN PARIETOCHLORIS INCISA (CHLOROPHYTA, TREBOUXIOPHYCEAE) MUTANT DEFICIENT IN Δ5 DESATURASE BY NITROGEN STARVATION AND HIGH LIGHT 1
TLDR
The P127 mutant responded to the stresses by coordinated induction of fatty acid and carotenoid syntheses, but displayed the same magnitude of the response as was observed in wildtype under 30% lower irradiance, making it possible to develop a nondestructive technique for the assay of TFA and DGLA.
Phylogenetically-based variation in the regulation of the Calvin cycle enzymes, phosphoribulokinase and glyceraldehyde-3-phosphate dehydrogenase, in algae.
TLDR
The diversity of redox-regulation of phosphoribulokinase and glyceraldehyde-3-phosphate dehydrogenase was investigated in a wide range of algal groups under standard conditions, highlighting the importance of understanding enzyme regulation in diverse algae if their ecology and productivity is to be understood.
LIPID SYNTHESIS AND FATTY ACID COMPOSITION IN NANNOCHLOROPSIS SP. (EUSTIGMATOPHYCEAE) GROWN IN A LIGHT‐DARK CYCLE 1
TLDR
The cellular content of chlorophyll a and carotenoids increased during the light period and decreased during the dark period, and cellular components, such as proteins, carbohydrates and lipids, followed a similar pattern.
...
1
2
3
4
5
...