De Novo Generated Human Red Blood Cells in Humanized Mice Support Plasmodium falciparum Infection

@article{Amaladoss2015DeNG,
  title={De Novo Generated Human Red Blood Cells in Humanized Mice Support Plasmodium falciparum Infection},
  author={Anburaj Amaladoss and Qingfeng Chen and Min Liu and Sara K. Dummler and Ming Dao and Subra Suresh and Jianzhu Chen and Peter Rainer Preiser},
  journal={PLoS ONE},
  year={2015},
  volume={10}
}
Immunodeficient mouse–human chimeras provide a powerful approach to study host specific pathogens like Plasmodium (P.) falciparum that causes human malaria. Existing mouse models of P. falciparum infection require repeated injections of human red blood cells (RBCs). In addition, clodronate lipsomes and anti-neutrophil antibodies are injected to suppress the clearance of human RBCs by the residual immune system of the immunodeficient mice. Engraftment of NOD-scid Il2rg-/- mice with human… 
Humanized Mice Are Instrumental to the Study of Plasmodium falciparum Infection
TLDR
This work reviews the strategies employed to overcome the remaining-limitations of the developed human-mouse chimera(s) and discusses the process of “humanization” of various immunodeficient/transgenic strains and their contribution to translational biomedical research.
Plasmodium falciparum Liver Stage Infection and Transition to Stable Blood Stage Infection in Liver-Humanized and Blood-Humanized FRGN KO Mice Enables Testing of Blood Stage Inhibitory Antibodies (Reticulocyte-Binding Protein Homolog 5) In Vivo
TLDR
Test whether a monoclonal antibody targeting the erythrocyte invasion ligand reticulocyte-binding protein homolog 5 was capable of blocking blood stage infection in vivo when parasites emerge from the liver was found to be highly effective and found it highly effective.
Modeling Infectious Diseases in Mice with a "Humanized" Immune System.
TLDR
Compared to nonhumanized animal models, the HIS mouse models allow the analysis of interactions between human immune cells and bona fide pathogenic microbes, making them a more clinically relevant model.
Humanized Mouse Models for the Study of Human Malaria Parasite Biology, Pathogenesis, and Immunity
TLDR
Current humanized mouse models and the future directions that should be taken to develop next-generation models for human malaria parasite research are discussed.
A Hitchhiker's guide to humanized mice: new pathways to studying viral infections
TLDR
A detailed summary of the generation of human peripheral blood lymphocyte‐, CD34+ haematopoietic stem cell‐ and bone marrow/liver/thymus‐reconstituted mice and available improved models and future perspectives for their use in biomedical, drug and vaccine research are summarized.
Hematological Humanization of Immune-Deficient Mice.
TLDR
The method to humanize immune-deficient mice with human CD34+ hematopoietic cells is described and the growing field of immune-oncology could benefit from preclinical studies with the humanized mice.
Humanized Mouse Models of Staphylococcus aureus Infection
  • D. Parker
  • Biology, Medicine
    Front. Immunol.
  • 2017
TLDR
Questions are raised as to the utility of mice to predict patient outcome and the development of humanized mouse models of S. aureus infection are suggested to suggest that humanized mice might prove useful in modeling infection.
Developing a xenograft model of human vasculature in the mouse ear pinna
TLDR
This model represents a useful tool for imaging the interactions that occur within human tissues in vivo and permits visualization of blood flow and cellular recruitment in a system which is amenable to intervention for various studies in basic biology together with drug evaluation and mechanism of action studies.
Developing a xenograft model of human vasculature in the mouse ear pinna
TLDR
This model represents a useful tool for imaging the interactions that occur within human tissues in vivo and permits visualization of blood flow and cellular recruitment in a system which is amenable to intervention for various studies in basic biology together with drug evaluation and mechanism of action studies.
Humanized Mice as Unique Tools for Human-Specific Studies
TLDR
This review will focus on the background of humanized mice, diseases, and human-specific therapeutics tested on this platform as well as solutions to improve humanized mouse models for future clinical use.
...
1
2
3
...

References

SHOWING 1-10 OF 30 REFERENCES
Macrophages prevent human red blood cell reconstitution in immunodeficient mice.
TLDR
The data demonstrate that human RBCs are highly susceptible to rejection by macrophages in immunodeficient mice, and strategies for preventing human R BC rejection bymacrophages are required for using immunodecisive mice as an in vivo model to study human erythropoiesis and RBC function.
Human natural killer cells control Plasmodium falciparum infection by eliminating infected red blood cells
TLDR
RBC-supplemented, immune cell-optimized humanized (RICH) mice that support multiple cycles of P. falciparum infection are developed and it is shown that the adhesion molecule lymphocyte-associated antigen 1 is required for NK cell interaction with and elimination of iRBCs.
Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice
TLDR
Human cytokine gene expression by hydrodynamic delivery is a simple and efficient method to improve reconstitution of specific human-blood cell lineages in humanized mice, providing an important tool for studying human immune responses and disease progression in a small animal model.
A Murine Model of falciparum-Malaria by In Vivo Selection of Competent Strains in Non-Myelodepleted Mice Engrafted with Human Erythrocytes
TLDR
Using this model, a reproducible assay of antimalarial activity useful for drug discovery is implemented and validated and demonstrates that P. falciparum contains clones able to grow reproducibly in mice engrafted with human erythrocytes without the use of myeloablative methods.
Maintenance of the human malarial parasite, Plasmodium falciparum, in scid mice and transmission of gametocytes to mosquitoes
TLDR
These results are the first demonstration of human malarial parasite propagation in mice and transmission of these parasites to the invertebrate vector.
Human Malaria in Immunocompromised Mice An in Vivo Model to Study Defense Mechanisms against Plasmodium falciparum
TLDR
The results show that the total African adult hyperimmune immunoglobulin Gs (HI-IgGs) strongly reduce P. falciparum parasitemia similarly to that reported in humans, but only when mice are concomitantly reconstituted with human monocytes (HuMNs).
Further Improvements of the P. falciparum Humanized Mouse Model
TLDR
The role of aging, of inosine and of the IL-2 receptor γ mutation in controlling P. falciparum induced inflammation is demonstrated and the combination of the NSG mouse, clodronate loaded liposomes, and IV delivery of huRBC has produced a reliable and more relevant model that better meets the needs of Malaria research.
Human Lymphoid and Myeloid Cell Development in NOD/LtSz-scid IL2Rγnull Mice Engrafted with Mobilized Human Hemopoietic Stem Cells 12
TLDR
NOD-scid IL2Rγnull mice engrafted with human mobilized blood stem cells provide a new in vivo long-lived model of robust multilineage human HSC engraftment.
Human Malaria in Immunocompromised Mice: New In Vivo Model for Chemotherapy Studies
TLDR
Data suggest that P. falciparum-huRBC–BXN mice can provide a valuable in vivo system and should be included in the short list of animals that can be used for the evaluation of P. Falconerum responses to drugs.
...
1
2
3
...