Dbf4-Cdc7 phosphorylation of Mcm2 is required for cell growth.

  title={Dbf4-Cdc7 phosphorylation of Mcm2 is required for cell growth.},
  author={Irina Bruck and Daniel L Kaplan},
  journal={The Journal of biological chemistry},
  volume={284 42},
The Dbf4-Cdc7 kinase (DDK) is required for the activation of the origins of replication, and DDK phosphorylates Mcm2 in vitro. We find that budding yeast Cdc7 alone exists in solution as a weakly active multimer. Dbf4 forms a likely heterodimer with Cdc7, and this species phosphorylates Mcm2 with substantially higher specific activity. Dbf4 alone binds tightly to Mcm2, whereas Cdc7 alone binds weakly to Mcm2, suggesting that Dbf4 recruits Cdc7 to phosphorylate Mcm2. DDK phosphorylates two… CONTINUE READING

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