Daunorubicin metabolism: estimation of daunorubicin reductase.

  title={Daunorubicin metabolism: estimation of daunorubicin reductase.},
  author={Nicholas R. Bachur and David H. Huffman},
  journal={British journal of pharmacology},
  volume={43 4},
1. Daunorubicin reductase, an enzyme ubiquitous in all mammalian tissues tested, converts daunorubicin, a cancer chemotherapeutic antibiotic, to daunorubicinol. A method for the estimation of daunorubicinol and daunorubicin reductase is described.2. Daunorubicin and daunorubicinol were quantitatively extracted from enzyme assay mixtures with 0.3 N HCl in 50% ethanol and then completely hydrolysed to their respective aglycones. The aglycones were extracted, concentrated into toluene, separated… CONTINUE READING