Database interrogation algorithms for identification of proteins in proteomic separations.

@article{Palagi2009DatabaseIA,
  title={Database interrogation algorithms for identification of proteins in proteomic separations.},
  author={Patricia M. Palagi and Fr{\'e}d{\'e}rique Lisacek and Ron D. Appel},
  journal={Methods in molecular biology},
  year={2009},
  volume={519},
  pages={515-31}
}
Protein identification is a key aspect in the investigation of proteomes. Typically, in a 2-DE gel-based proteomics analysis, the spots are enzymatically digested and the resulting peptide masses are measured, producing mass spectra. Peptides can also be isolated and fragmented within the mass spectrometer, leading to tandem mass spectra. For protein and peptide identification, an algorithm matches the mass spectra and other empirical information against a protein database to define if a… CONTINUE READING