Darkness and antibiotics increase the steady-state transcripts of the elongation factor gene (tuf) inChlamydomonas reinhardtii

@article{Silk1988DarknessAA,
  title={Darkness and antibiotics increase the steady-state transcripts of the elongation factor gene (tuf) inChlamydomonas reinhardtii},
  author={Gregg W. Silk and Madeline C. S. Wu},
  journal={Current Genetics},
  year={1988},
  volume={14},
  pages={119-126}
}
A plasmid library of chloroplast (Cp) DNA fromChlamydomonas reinhardtii was used to screen for transcripts which respond to light. A transcript of R03, a 1,300 byEcoRI fragment, was identified as a message which accumulates in darkness. The transcribed region of R03 showed extensive sequence homology with theEscherichia coli elongation factor gene,tufA. A genespecific probe was constructed. Northern blots were used to study the extent and kinetics of accumulation of this transcript in darkness… CONTINUE READING

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