Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum.

@article{Tsai1990DarkinducedAO,
  title={Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum.},
  author={F.-Y. Tsai and Gloria M. Coruzzi},
  journal={The EMBO journal},
  year={1990},
  volume={9 2},
  pages={323-32}
}
Nucleotide sequence analysis of cDNAs for asparagine synthetase (AS) of Pisum sativum has uncovered two distinct AS mRNAs (AS1 and AS2) encoding polypeptides that are highly homologous to the human AS enzyme. The amino-terminal residues of both AS1 and AS2 polypeptides are identical to the glutamine-binding domain of the human AS enzyme, indicating that the full-length AS1 and AS2 cDNAs encode glutamine-dependent AS enzymes. Analysis of nuclear DNA shows that AS1 and AS2 are each encoded by… CONTINUE READING