Dansyl-galactoside, a fluorescent probe of active transport in bacterial membrane vesicles.

@article{Reeves1973DansylgalactosideAF,
  title={Dansyl-galactoside, a fluorescent probe of active transport in bacterial membrane vesicles.},
  author={John P. Reeves and Evan Shechter and R. Weil and H Ronald Kaback},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1973},
  volume={70 10},
  pages={2722-6}
}
A fluorescent galactoside, 2-(N-dansyl)-aminoethyl beta-D-thiogalactoside (dansyl-galactoside), competitively inhibits lactose transport by membrane vesicles of Escherichia coli, but is not actively transported. An increase in dansyl-galactoside fluorescence is observed upon addition of D-lactate. The fluorescence increase is not observed in membrane vesicles lacking the beta-galactoside transport system, and is blocked or rapidly reversed by addition of beta-galactosides, sulfhydryl reagents… CONTINUE READING

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Current Topics in Bioenergetics, ed

  • S. Murakawa, K. Izaki, H. Takahashi
  • Agr. Biol. Chem
  • 1973

Biochim. Biophys

  • H. R. Kaback
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