Dansyl-galactoside, a fluorescent probe of active transport in bacterial membrane vesicles.

  title={Dansyl-galactoside, a fluorescent probe of active transport in bacterial membrane vesicles.},
  author={John P. Reeves and Evan Shechter and R. Weil and H Ronald Kaback},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={70 10},
A fluorescent galactoside, 2-(N-dansyl)-aminoethyl beta-D-thiogalactoside (dansyl-galactoside), competitively inhibits lactose transport by membrane vesicles of Escherichia coli, but is not actively transported. An increase in dansyl-galactoside fluorescence is observed upon addition of D-lactate. The fluorescence increase is not observed in membrane vesicles lacking the beta-galactoside transport system, and is blocked or rapidly reversed by addition of beta-galactosides, sulfhydryl reagents… CONTINUE READING


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