Damaged DNA-binding protein DDB stimulates the excision of cyclobutane pyrimidine dimers in vitro in concert with XPA and replication protein A.

@article{Wakasugi2001DamagedDP,
  title={Damaged DNA-binding protein DDB stimulates the excision of cyclobutane pyrimidine dimers in vitro in concert with XPA and replication protein A.},
  author={Mitsuo Wakasugi and Mamoru Shimizu and Hiroshi Morioka and S. Linn and Osamu Nikaido and Tsukasa Matsunaga},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 18},
  pages={15434-40}
}
Human cells contain a protein that binds to UV-irradiated DNA with high affinity. This protein, damaged DNA-binding protein (DDB), is a heterodimer of two polypeptides, p127 and p48. Recent in vivo studies suggested that DDB is involved in global genome repair of cyclobutane pyrimidine dimers (CPDs), but the mechanism remains unclear. Here, we show that in vitro DDB directly stimulates the excision of CPDs but not (6-4)photoproducts. The excision activity of cell-free extracts from Chinese… CONTINUE READING