Damage to chromosomes and DNA of rhesus monkey sperm following cryopreservation.

@article{Li2007DamageTC,
  title={Damage to chromosomes and DNA of rhesus monkey sperm following cryopreservation.},
  author={M W Li and Stuart A. Meyers and Theodore L. Tollner and James W. Overstreet},
  journal={Journal of andrology},
  year={2007},
  volume={28 4},
  pages={493-501}
}
Fresh and frozen-thawed rhesus monkey sperm were analyzed for DNA damage using the comet assay and for chromosome damage by cytogenetic analysis after intracytoplasmic sperm injection (ICSI) into mouse oocytes. The percentage of fresh sperm with damaged DNA in ejaculated semen was 0 to 2.7% (n = 5). Conventional cryopreservation and storage in liquid nitrogen caused DNA damage in 25.3% to 43.7% of sperm; when sperm were frozen without cryoprotectants, 52.7% to 92.0% of thawed sperm had DNA… CONTINUE READING