Damage and regeneration of hair cell ciliary bundles in a fish ear following treatment with gentamicin

@article{Lombarte1993DamageAR,
  title={Damage and regeneration of hair cell ciliary bundles in a fish ear following treatment with gentamicin},
  author={Antoni Lombarte and Hong Young Yan and Arthur N. Popper and Janet S. Chang and Christopher Platt},
  journal={Hearing Research},
  year={1993},
  volume={64},
  pages={166-174}
}
Sensory hair cells in the striolar regions of the utricle and lagena of a teleost fish, the oscar (Astronotus ocellatus), were damaged following intramuscular injections of gentamicin sulfate. In order to determine whether fish can regenerate hair cells, the time course of damage and recovery was followed over a period of four weeks by scanning electron microscopy. Maximum loss of ciliary bundles occurred at about day 10 after the first of four daily injections of gentamicin (20 mg/kg) in 4-6… Expand
View on Elsevier
doi.org
130 Citations
Highly Influential Citations
8
Background Citations
54
Methods Citations
1
Results Citations
1

130 Citations

Citation Type

Citation Type

Distribution and time course of hair cell regeneration in the pigeon utricle
TLDR
In the present study, intraotic application of a 2 mg streptomycin paste was used to achieve complete vestibular hair cell destruction in pigeons (Columba livia) while preserving regenerative ability. Expand
Cell proliferation follows acoustically-induced hair cell bundle loss in the zebrafish saccule
TLDR
A time course of hair cell bundle regeneration in the zebrafish inner ear is established and it is demonstrated that cell proliferation is associated with the regenerative process. Expand
Damage and recovery of hair cells in fish canal (but not superficial) neuromasts after gentamicin exposure
TLDR
The results suggest that the hair cells in the canal and superficial neuromasts may be similar to type I-like and type II hair cells, respectively, in the fish ear. Expand
Sensory hair cell death and regeneration in fishes
TLDR
Advances derived from studies in zebrafish and other fish species include understanding the effect of ototoxins on hair cells and finding otoprotectants to mitigate ototoxin damage, and elucidating cellular pathways involved in the regeneration process. Expand
A brief history of hair cell regeneration research and speculations on the future
TLDR
The field of hair cell regeneration has continued to investigate the capacity of the auditory and vestibular epithelia in vertebrates to regenerate hair cells and to recover function, the molecular mechanisms governing these regenerative capabilities, and the prospect of designing biologically-based treatments for hearing loss and balance disorders. Expand
Proliferative Regeneration of Zebrafish Lateral Line Hair Cells after Different Ototoxic Insults
Sensory hair cells in the zebrafish lateral line regenerate rapidly and completely after damage. Previous studies have used a variety of ototoxins to kill lateral line hair cells to study different… Expand
Hair Cell Regeneration in the Inner Ear
  • E. Rubel, T. Tsue, E. Oesterle, E. Rubel
  • Biology, Medicine
  • Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery
  • 1994
TLDR
Optimism is yielded that direct stimulation of hair cell production or transplantation of living hair cells may eventually become treatment modalities for the damaged human inner ear. Expand
Apoptosis and hair cell degeneration in the vestibular sensory epithelia of the guinea pig following a gentamicin insult
TLDR
The results indicated that vestibular hair cells undergo apoptosis after ototoxic traumas, a mode of hair cell loss in mammalian inner ear sensory epithelia. Expand
Damage and functional recovery of the Atlantic cod (Gadus morhua) inner ear hair cells following local injection of gentamicin
TLDR
It is suggested that the macula sacculi of Atlantic cod can regenerate towards a near-complete functional and ultrastructural recovery within 17–21 days following ototoxic gentamicin treatment. Expand
Two modes of hair cell loss from the vestibular sensory epithelia of the guinea pig inner ear
TLDR
Two distinct modes of hair cell loss were identified: degeneration of hair cells within the epithelium, which often showed morphological features consistent with those described for apoptosis, and extrusion of intact cells from the apical surface. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 28 REFERENCES
Light microscopic evidence of hair cell regeneration after gentamicin toxicity in chick cochlea.
TLDR
There appears to be a progressive partial recovery of the normal hair cell counts in the 25- and 32-day-old animals, whereas in the control chicks, there was a 36% hair cell loss. Expand
Regeneration of hair cell stereociliary bundles in the chick cochlea following severe acoustic trauma
Examination of pure-tone acoustic damage in the chick cochlea revealed a significant amount of hair cell recovery over a 10 day period following the exposure. The recovery included both a… Expand
Growth of a fish ear: 1. Quantitative analysis of hair cell and ganglion cell proliferation
TLDR
The number of sensory hair cells on the saccular sensory epithelium in specimens of the cichlid fish Astronotus ocellatus and the increase in both length and weight of the animals studied were statistically correlated and the average ratio of hair cells to afferent fibers increases from about 30:1 in the smallest fish to over 300: 1 in the largest animals. Expand
Hair Cell Loss and Regeneration after Exposure to Intense Sound in Neonatal Chicks
TLDR
Hair-cell restoration after severe acoustic injury from intense sound exposure in the neonatal ear is demonstrated and can be recognized by the disorganization of hair cells in the previously injured area. Expand
Hair cell regeneration in the chicken cochlea following aminoglycoside toxicity
TLDR
Results show that the restoration of hair cell number following aminoglycoside toxicity results from the production of new cells by mitosis, similar to developing auditory hair cells in avian embryos. Expand
Hair cell damage and recovery following chronic application of kanamycin in the chick cochlea
TLDR
The base-to-apex gradient of morphological development along the cochlea was observed in the process of regeneration and Kinocilium and microvilli were observed on the apical surfaces of the regenerating hair cells. Expand
Possible precursors of regenerated hair cells in the avian cochlea following acoustic trauma
TLDR
Examination of animals in short survival groups suggest that two precursor populations for regenerating hair cells may exist, and cuboidal or hyaline epithelial cells appear to serve as the precursor cell population for the regeneration of both hair cells and supporting cells. Expand
Hair cell regeneration in the adult budgerigar after kanamycin ototoxicity
TLDR
Although the appearance of the basilar papilla had improved considerably by 28 days post-treatment, the sensory epithelium still contained a number of pathologies, most noticeably, incomplete restoration of hair cell number in the most apical part of the damaged region and the disorganization ofhair cell packing. Expand
Ultrastructural observations on regenerating hair cells in the chick basilar papilla
TLDR
Investigation of cellular and subcellular maturational changes in regenerated immature sensory cells and support cells of the chick basilar papilla following gentamycin treatment suggested that determination of the cellular phenotype occurred at the level of the basal membrane followed by migration to the surface, during which time differentiation was characterized by an increase in number and complexity of cellular organelles. Expand
Regeneration in the auditory system
TLDR
Definitive identification of the progenitors of regenerated hair cells in the avian cochlea awaits further investigation, but evidence that points to two possible candidate cell types is discussed. Expand
...
1
2
3
...