Daily Rhythm of Luciferin Activity in Gonyaulax polyedra

@article{Bode1963DailyRO,
  title={Daily Rhythm of Luciferin Activity in Gonyaulax polyedra},
  author={Vernon C. Bode and R Desa and John Woodland Hastings},
  journal={Science},
  year={1963},
  volume={141},
  pages={913 - 915}
}
Extracts prepared from Gonyaulax cells harvested late in the 12-hour dark period of an artificial 24-hour day contain as much as four times more luciferin activity than similar extracts prepared during the light period. If cultures are not treated with heat or light to reduce the flashing associated with handling, the time of maximum activity is obscured. 

Circadian rhythms: mechanism of luciferase activity changes in Gonyaulax.

TLDR
The immediate biochemical basis of the luciferase rhythm was investigated, and Mixing experiments, ammonium sulfate precipitation, dialysis, and sucrose velocity gradient centrifugation showed that no dissociable activator or inhibitor of Luciferase caused the rhythm.

Circadian spontaneous bioluminescent glow and flashing ofGonyaulax polyedra

TLDR
A new, fully computerized method for the measurement and analysis of dinoflagellate bioluminescence has been developed and applied to the spontaneous light emission of Gonyaulax polyedra, suggesting that the controls for the two processes must at some point diverge.

Sensitivity to stimulation, a component of the circadian rhythm in luminescence in gonyaulax.

TLDR
The differences in threshold and number of flahses are evidence for a second component of the circadian rhythm in luminescence, a rhythm in sensitivity to stimulation in dinoflagellate Gonyaulax polyedra.

Phased Protein Synthesis at Several Circadian Times Does Not Change Protein Levels in Gonyaulax

TLDR
No substantial changes were found in the levels of any other rhythmically synthesized proteins examined, unlike the circadian changes in the synthesis rate of the luciferin binding protein, which produces variations in protein levels that correlate with the bioluminescence rhythm.

Influence of light regimen on the growth and toxicity of Alexandrium tamarense.

TLDR
The cage-culture turbidostat allows phytoplankton to be maintained in a constant chemical environment for extended periods, and growth rate to be easily determined, and the hypothesis that the toxin content of the cells was inversely proportional to their growth rate was investigated.

THE CIRCADIAN RHYTHM OF BIOLUMINESCENCE IN PYROCYSTIS IS NOT DUE TO DIFFERENCES IN THE AMOUNT OF LUCIFERASE: A COMPARATIVE STUDY OF THREE BIOLUMINESCENT MARINE DINOFLAGELLATES

TLDR
In the Pyrocystis species, the amount of luciferase is the same in extracts made during the day and night phases; its circadian regulation in vivo may be attributed to a change in its localization from day to night phase.

Solubilization of molecular elements of scintillons: Bioluminescent particles from dinoflagellates

TLDR
The results suggest that the substrate-binding protein is bound to the particle chiefly by electrostatic forces, whereas the attachment of luciferase involves instead hydrophobic interactions, and that the two are present in the particles in approximately equal amounts.

References

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A PERSISTENT DIURNAL RHYTHM OF LUMINESCENCE IN GONYAULAX POLYEDRA

TLDR
The characteristics of a persistent diurnal rhythm of luminescence in the dinoflagellate Gonyaulax polyedra are described and Cultures which have been grown in bright light for as long as one year show a diurnal Rhythmicity when placed in constant dim light or darkness.

BIOCHEMISTRY OF RHYTHMIC SYSTEMS *

TLDR
A particular biological rhythm observed is concluded to be a spontaneous and truly endogenous rhythm set up by the root, and the methods used to throw some light on the interactions through which it is generated.

ON ENZYME-SUBSTRATE RELATIONSHIPS DURING BIOCHEMICAL DIFFERENTIATION.

  • B. Wright
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1960
TLDR
21 Stent, G. S., J. D., and F. R. Astrachan, in The Chemical Basis of Heredity, loc.

The luminescent reaction in extracts of the marine dinoflagellate, Gonyaulax polyedra.

This research was supported in part by U.S. Public Health Service grants A-2462(C2) and GM-7009(C4), and Atomic Energy Commission grant AT