Dipeptidyl peptidase-4 (DPP-4 or CD26) inhibitors, a new class of antidiabetic compounds, are effective in the treatment of hyperglycemia. Because atherosclerosis-related cardiovascular diseases are the major complications of diabetes, it is important to determine the effect of DPP-4 inhibitors on atherosclerosis. In this study, nondiabetic and diabetic… (More)
FIGURE 5. Inhibition of LPS-stimulated proinflammatory cytokine secretion and mRNA expression in U937 cells by alogliptin. A, C, U937 cells were treated with or without 10 ng/mL of LPS in the absence or the presence of different concentrations of alogliptin for 24 hours. After the treatment, IL-6 (A) and IL-1b (C) in the culture medium were quantified using ELISA. B, U937 cells were treated with or without 10 ng/mL of LPS in the absence or the presence of 1 nM of alogliptin for 24 hours. After the treatment, cellular IL-6 mRNA was quantified using real-time PCR. The expression of IL-6 mRNA was normalized to that of glyceraldehyde 3-phosphate dehydrogenase mRNA.