DOGR1 and DOGR2: two genes from Saccharomyces cerevisiae that confer 2-deoxyglucose resistance when overexpressed.

@article{RndezGil1995DOGR1AD,
  title={DOGR1 and DOGR2: two genes from Saccharomyces cerevisiae that confer 2-deoxyglucose resistance when overexpressed.},
  author={Francisca R{\'a}ndez-Gil and Amalia Blasco and Jos{\'e} Antonio Fern{\'a}ndez Prieto and Pascual Sanz},
  journal={Yeast},
  year={1995},
  volume={11 13},
  pages={1233-40}
}
Saccharomyces cerevisiae contains two genes (DOGR1 and DOGR2) that are able to confer 2-deoxyglucose resistance when they are overexpressed. These genes are very similar, sharing 92% identity at the protein level. They code for two isoenzymes with 2-deoxyglucose-6 phosphate (2-DOG-6P) phosphatase activity. These enzymes have been purified and characterized. DogR1p shows an optimum pH of 6, an optimum temperature of 30 degrees C and a KM on 2-DOG-6P of 17 mM. DogR2p shows a similar optimum pH… CONTINUE READING

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