DNaseI footprinting studies of Escherichia coli biotin repressor-operator interactions.

@article{Lin1993DNaseIFS,
  title={DNaseI footprinting studies of Escherichia coli biotin repressor-operator interactions.},
  author={K. Lin and D. Shiuan},
  journal={Journal of biochemistry},
  year={1993},
  volume={114 5},
  pages={
          670-6
        }
}
  • K. Lin, D. Shiuan
  • Published 1993
  • Biology, Medicine
  • Journal of biochemistry
  • Transcription of Escherichia coli biotin operon is repressed by biotin repressor in the presence of biotinyl-5'-adenylate as a corepressor. To determine precisely the site of action of biotin repressor on the operator sequence, DNaseI footprinting experiments were performed on the PCR-produced biotin operator and its mutants. The results indicate that the repressor binds to the wild-type operator as well as mutated operator sequence at +15 position or -15 position, and protects the 39-base… CONTINUE READING
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