DNase production by Clostridium septicum

@article{Swiatek1987DNasePB,
  title={DNase production by Clostridium septicum},
  author={P. Swiatek and S. Allen and J. Siders and C. Lee},
  journal={Journal of Clinical Microbiology},
  year={1987},
  volume={25},
  pages={437 - 438}
}
Sixty-two Clostridium septicum isolates were assayed for extracellular DNase activity. All of the C. septicum isolates tested produced greater DNase activity than did the other DNase-producing clostridial isolates tested. The molecular weight of the DNase of C. septicum was determined to be approximately 45,000. DNase is a major extracellular protein produced by this organism. 
MLST analysis reveals a highly conserved core genome among poultry isolates of Clostridium septicum.
TLDR
Phylogenetic analyses identified two unique clusters, or clonal complexes, among these poultry isolates which may have important epidemiological implications for poultry producers in the United States. Expand
Toxin Synthesis and Mucin Breakdown Are Related to Swarming Phenomenon in Clostridium septicum
TLDR
Environmental and physiologic factors affecting growth and swarm cell formation in C. septicum were studied over a range of dilution rates, showing that swarming occurred in response to nutrient depletion. Expand
A Rapid Protocol for Preparation of Clostridium septicum Genomic DNA
TLDR
This method circumvents the earlier tedious purification procedures involving cesium chloride/Ethidium bromide density gradient centrifugation and yields genomic DNA of good quality and integrity. Expand
In silico, in vitro and in vivo analysis of putative virulence factors identified in large clostridial toxin-negative, binary toxin producing C. difficile strains.
The relevance of large clostridial toxin-negative, binary toxin-producing (A-B-CDT+) Clostridium difficile strains in human infection is still controversial. In this study, we investigated putativeExpand
Toxigenic clostridia
TLDR
Toxigenic clostridia belonging to 13 recognized species are discussed in this review, using the term toxin to include the various antigenic protein products of these organisms with biological and serological activities which have served as distinguishing characteristics for differentiation and classification. Expand
Genomic Analysis of Clostridium botulinum Group II by Pulsed-Field Gel Electrophoresis
TLDR
The results substantiate that after problems due to DNases are overcome, PFGE analysis will be a reproducible and highly discriminating epidemiological method for studying C. botulinum group II at the molecular level. Expand
Molecular characterization of the diversity of Clostridium chauvoei isolates collected from two bovine slaughterhouses: analysis of cross-contamination.
TLDR
The preliminary results suggest that REA combined with numerical analysis provides additional criteria and characteristic banding patterns for the study of the cross-contamination and characterization of C. chauvoei. Expand
Identification of factors affecting the high pressure tolerance of Clostridium botulinum type E endospores
TLDR
The probing of different transformation strategies suggested that C. botulinum (beluga) possess an effective restriction endonuclease, which prevents the uptake of foreign DNA and hinders the construction of knock out mutants for detailed analyses. Expand
Isolation and characterization of DNase-deficient mutants ofClostridium acetobutylicum
DNase activity ofClostridium acetobutylicum was found to exhibit a relatively broad, acidic pH optimum of 3.5–4.5. The enzyme could be completely inhibited by addition of 0.2M EDTA (finalExpand
Trusting Your Gut: Diagnosis and Management of Clostridium septicum Infections
TLDR
A summary of the current knowledge related to the pathogen is presented, highlighting clinical manifestations, molecular pathogenesis, and the diagnostic significance of C. septicum in the clinical setting. Expand
...
1
2
...

References

SHOWING 1-9 OF 9 REFERENCES
COMPLEXITY OF TOXINS FROM CLOSTRIDIUM SEPTICUM AND CLOSTRIDIUM CHAUVOEI
TLDR
In attempting to study the toxins of C. septicum and C. chauvoei and to elucidate some of the relationships between their components, a number of toxigenic strains was investigated under different cultural and environmental conditions. Expand
Comparative study of streptococcal DNase by rapid and quantitative assay systems.
TLDR
It is suggested that this newly established assay system for DNase could be applicable for the rapid measurement of DNase activity to distinguish group A streptococci from the other groups. Expand
PARALLELISM IN THE LETHAL AND HEMOLYTIC ACTIVITY OF THE TOXIN OF CLOSTRIDIUM SEPTICUM
The relation of the lethal toxin to the hemolysin produced by Clostridium septicum, strain 44, has been investigated. The following results suggest that the hemolytic and lethal actions of crudeExpand
Clostridium septicum Infection and Malignancy
TLDR
Increased awareness of this association between C. septicum and malignancy, and aggressive surgical treatment, may result in improvement in the present 50–70% mortality rate. Expand
Anaerobe Laboratory manual
TLDR
The anaerobe laboratory manual is one of the literary work in this world in suitable to be reading material and it will show the amazing benefits of reading a book. Expand
Clostridium
  • Manual of clinical microbiology
  • 1985
Clostridium, p. 434-444
  • Manual of clinical microbiology,
  • 1985
Laboratory methods in anaerobic bacteriology. Center for Disease Control laboratory manual
  • 1977
THE PATHOGENIC ANAEROBIC BACTERIA