DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.

Abstract

DNase I pretreatment of 16S rRNA gene PCR reagents was tested. The DNase I requirement for the elimination of false-positive results varied between 0.1 and 70 IU per master mix depending on the applied Taq polymerase. PCR sensitivity was mostly maintained when 0.1 IU of DNase I was used.

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@article{Heininger2003DNasePO, title={DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.}, author={Alexandra Heininger and Marlies Binder and Andreas Ellinger and Konrad Botzenhart and Klaus E Unertl and Gerd Doering}, journal={Journal of clinical microbiology}, year={2003}, volume={41 4}, pages={1763-5} }