DNA vaccination with plasmids containing various fragments of large segment genome of infectious bursal disease virus.

@article{Chang2003DNAVW,
  title={DNA vaccination with plasmids containing various fragments of large segment genome of infectious bursal disease virus.},
  author={H C Chang and Tsang Long Lin and Ching-Ching Wu},
  journal={Vaccine},
  year={2003},
  volume={21 5-6},
  pages={507-13}
}
The present study was undertaken to determine the effectiveness in including VP2 gene of the large segment genome of infectious bursal disease virus (IBDV) in the DNA vaccine for protection of chickens against infectious bursal disease (IBD). Different fragments of the large segment gene of IBDV standard challenge (STC) strain were successfully amplified by reverse transcription-polymerase chain reaction (RT-PCR) followed by cloning into a eukaryotic expression plasmid vector (pCR3.1) as DNA… CONTINUE READING