DNA topoisomerases: structure, function, and mechanism.
@article{Champoux2001DNATS, title={DNA topoisomerases: structure, function, and mechanism.}, author={James J. Champoux}, journal={Annual review of biochemistry}, year={2001}, volume={70}, pages={ 369-413 } }
DNA topoisomerases solve the topological problems associated with DNA replication, transcription, recombination, and chromatin remodeling by introducing temporary single- or double-strand breaks in the DNA. In addition, these enzymes fine-tune the steady-state level of DNA supercoiling both to facilitate protein interactions with the DNA and to prevent excessive supercoiling that is deleterious. In recent years, the crystal structures of a number of topoisomerase fragments, representing nearly…
2,346 Citations
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This review focuses on SUMOylation of topoisomerases, which has been demonstrated to be a key modification of both type I and type II topoisomersases, and special emphasis is placed on recent studies that indicate how SUMoylation regulates topoisomease function in unperturbed cells and the unique roles that SUMOolation plays in repairing damage arising from topoisomase malfunction.
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This paper introduced topoisomerase inhibitors, mechanism of action and types of them, which are enzymes that control the changes in DNA structure by catalyzing the breaking and rejoining of the phosphodiester backbone of DNA strands during the normal cell cycle.
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Evidence from biophysical studies for the dynamic conformations that are required for type IA topoisomerases to catalyze the change in the topology of the nucleic acid substrates is obtained.
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