DNA topoisomerase II can drive changes in higher order chromosome architecture without enzymatically modifying DNA.

@article{Bojanowski1998DNATI,
  title={DNA topoisomerase II can drive changes in higher order chromosome architecture without enzymatically modifying DNA.},
  author={Kozystof Bojanowski and Andrew Maniotis and Sergei Y. Plisov and Annette K. Larsen and Donald E. Ingber},
  journal={Journal of cellular biochemistry},
  year={1998},
  volume={69 2},
  pages={127-42}
}
Topoisomerase II has been suggested to play a major role in chromosome organization based on its DNA decatenating activity and its ability to mediate direct binding interactions between DNA and nuclear matrix. However, this latter point remains controversial. Here we address the question of whether the chromatin binding activity of Topoisomerase II is sufficient to modify chromosome form using whole mammalian chromosomes in vitro. Intact chromosomes were microsurgically removed from living… CONTINUE READING