DNA topoisomerase I from Mycobacterium smegmatis. An enzyme with distinct features.

  title={DNA topoisomerase I from Mycobacterium smegmatis. An enzyme with distinct features.},
  author={T. J. Bhaduri and T K Bagui and D Sikder and Valakunja Nagaraja},
  journal={The Journal of biological chemistry},
  volume={273 22},
A type I topoisomerase has been purified to homogeneity from Mycobacterium smegmatis. It is the largest single subunit enzyme of this class having molecular mass of 110 kDa. The enzyme is Mg2+ dependent and can relax negatively supercoiled DNA, catenate, and knot single-stranded DNA, thus having typical properties of type I topoisomerases. Furthermore, the enzyme makes single-stranded nicks and the 5'-phosphoryl end of the nicked DNA gets covalently linked with a tyrosine residue of the enzyme… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 22 extracted citations


Publications referenced by this paper.
Showing 1-6 of 6 references

Nucleic Acids Res

  • N. Vinay Kumar, U. Varshney
  • 1997
1 Excerpt

Trends Microbiol

  • C. J. Dorman, N. N. Bhriain
  • 1993

DNA Topology and Its Biological Effects

  • N. R. Cozzarelli, J. C. Wang
  • Cold Spring Harbor Laboratory,
  • 1990

Molecular Cloning: A Laboratory Manual, 2nd Ed

  • J. Sambrook, E. F. Fritsch, T. Maniatis
  • Cold Spring Harbor Laboratory,
  • 1989
2 Excerpts

Perspectives in Biochemistry (Neurath

  • K. Drlica, R. J. Franco
  • H., ed)
  • 1989
1 Excerpt

The Mycobacteria, A Sourcebook (Kubica

  • G. W. Lawrence
  • 1984
1 Excerpt

Similar Papers

Loading similar papers…