DNA synthesis inhibition and cell death associated with hydroxyurea teratogenesis in rat embryos.

@article{Scott1971DNASI,
  title={DNA synthesis inhibition and cell death associated with hydroxyurea teratogenesis in rat embryos.},
  author={William J. Scott and E. J. Ritter and J. Gerald Wilson},
  journal={Developmental biology},
  year={1971},
  volume={26 2},
  pages={
          306-15
        }
}
Cell cycle phase specificity of hydroxyurea and its effects on the cell kinetics in embryonic spinal cord.
TLDR
It was observed that HU inhibited DNA-synthesis in all S-phase cells for more than 4 hours and all cells damaged by HU did not start DNA-Synthesis again and became necrotic.
Biosynthesis of DNA, RNA and proteins by mouse embryos cultured in the presence of a teratogenic dose of chlorambucil.
TLDR
The effect of chlorambucil on the rates of DNA, RNA, and protein synthesis in mouse embryos was investigated using a system of whole embryo culture and a trend toward decreasing rates of protein synthesis at some time beyond 8 h was noted.
Cell degeneration and recovery of the fetal mammalian brain after a chemical insult.
TLDR
It is suggested that in the embryo the large amount of cellular debris is eliminated mainly by autolysis, partially by macrophages, and also to some extent by neighboring neuroepithelial cells.
Inhibition of RNA synthesis, a possible mode of the embryotoxic action of hydroxyurea
TLDR
Onset, extent and duration of the metabolic changes were measured after application of “non-teratogenic” and teratogenic doses of hydroxyurea (HU) and with this double-labelling technique it is possible to survey the effects of embryotoxic drugs on various pathways in mammalian embryos in vivo.
Cell Death and Reduced Proliferative Rate
TLDR
It becomes necessary to know the secondary consequences of cell death in a particular organ system so that the temporal and quantitative aspects of necrosis needed to produce malformation become explainable.
Investigation of the effect of hydroxyurea on the cell cycle and the development of necrosis in the embryonic CNS of mice.
TLDR
From the morphological findings it can be concluded that in the case of the S-phase-specific metabolic pathway, which is influenced by HU, the HU is primarily dealing with DNA synthesis.
Ultrastructural alterations in neuroepithelial cells of mouse embryos exposed to cytotoxic doses of hydroxyurea
TLDR
Developing neuroepithelial cells from the cranial region of neural folds were examined at various intervals after administration of teratogenic or embryolethal doses of HU to ICR/DUB mice on ninth day of gestation and images indicated that neighboring cells phagocytosed the cellular fragments derived from the HU affected cells.
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References

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Hydroxyurea: effects on Chinese hamster cells grown in culture.
TLDR
The lethal and inhibitory actions of the drug make it a useful synchronizing agent in asynchronous populations, even though the length of the S period may be slightly reduced thereafter, although inhibited cells are more sensitive to X-ray responses within a short time after the drug is removed.
THE ACTION OF HYDROXYUREA ON MOUSE L‐CELLS *
TLDR
The data presented in this communication taken together with observations of other workers would appear to suggest that the effect of the drug may be directly on the DNA molecule, and can be partially prevented by the addition of deoxyribonucleosides which in sufficient concentration appear to compete temporarily with the drug.
Teratogenesis and inhibition of DNA synthesis induced in rat embryos by cytosine arabinoside.
TLDR
Embryotoxic effects in 20-day fetuses were more closely related to the cumulative suppression of DNA synthesis caused by a given dosage than with either the level at any one posttreatment interval or the duration of the suppression.
Hydroxyurea: Differential Lethal Effects on Cultured Mammalian Cells during the Cell Cycle
TLDR
Hydroxyurea has a differential lethal effect on cultured Chinesehamster cells that are at different stages in their cell cycle, which may be useful for synchronizing asynchronous cell populations and explaining effects of the drug in human therapy.
The effect of Co60-radiation on development and DNA synthesis in the 11-day rat embryo.
  • R. Skalko
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    The Journal of experimental zoology
  • 1965
TLDR
DNA synthesis was suppressed in both series of irradiated embryos at 3, 6 and 12 hours after irradiation, the higher dose producing the larger amount of suppression, indicating that suppression of DNA synthesis reflected a change in the cellular population which is synthesizing DNA.
Action of hydroxyurea on the nucleic acid metabolism and viability of HeLa cells.
TLDR
Exposure of HeLa S-3 cells to 10 -3 m hydroxyurea for a period of 16 hr produced a loss of cell viability accompanied by an inhibition of DNA synthesis, which suggests an unbalanced growth similar to that previously observed with 5-fluorodeoxyuridine, excess thymidine, and 1-β-d-arabinofuranosylcytosine.
Hydroxyurea. I. Acute cell death in proliferating tissues in rats.
TLDR
The lethal effects of the hydroxamates are restricted to cells committed to DNA synthesis, and other cells in proliferating tissues escape damage during the limited time of circulation of inhibitory concentrations; this accounts for the prompt repair of tissue defects.
Differential effects of hydroxyurea on survival of proliferating cells in vivo.
TLDR
A single injection of hydroxyurea inhibits DNA synthesis in continuously dividing cells and in quiescent tissues that are stimulated to synthesize DNA and divide by an appropriate stimulus and seems capable of distinguishing DNA-synthesizing cells from different types of tissues.
The effects of hydroxyurea and related compounds on the rat fetus.
TLDR
The similarity in the spectra of abnormalities produced by hydroxyurea, hydroxyUREthan, and acetohydroxamic acid at biologically comparable doses suggest that these compounds may have a common mechanism of action.
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