DNA synapsis through transient tetramerization triggers cleavage by Ecl18kI restriction enzyme

@inproceedings{Zaremba2010DNAST,
  title={DNA synapsis through transient tetramerization triggers cleavage by Ecl18kI restriction enzyme},
  author={Mindaugas Zaremba and Amelia Owsicka and Gintautas Tamulaitis and Giedrius Sasnauskas and L. S. Shlyakhtenko and Alexander Y. Lushnikov and Yuri L Lyubchenko and Niels Laurens and Bram van den Broek and Gijs J. L. Wuite and Virginijus Siksnys},
  booktitle={Nucleic acids research},
  year={2010}
}
To cut DNA at their target sites, restriction enzymes assemble into different oligomeric structures. The Ecl18kI endonuclease in the crystal is arranged as a tetramer made of two dimers each bound to a DNA copy. However, free in solution Ecl18kI is a dimer. To find out whether the Ecl18kI dimer or tetramer represents the functionally important assembly, we generated mutants aimed at disrupting the putative dimer-dimer interface and analysed the functional properties of Ecl18kI and mutant… CONTINUE READING