DNA shuffling of subgenomic sequences of subtilisin

@article{Ness1999DNASO,
  title={DNA shuffling of subgenomic sequences of subtilisin},
  author={Jon E. Ness and M. Welch and Lori Giver and Manuel Mart{\'i}nez Bueno and Joel R. Cherry and Torben V. Borchert and Willem P. C. Stemmer and Jeremy Minshull},
  journal={Nature Biotechnology},
  year={1999},
  volume={17},
  pages={893-896}
}
DNA family shuffling of 26 protease genes was used to create a library of chimeric proteases that was screened for four distinct enzymatic properties. Multiple clones were identified that were significantly improved over any of the parental enzymes for each individual property. Family shuffling, also known as molecular breeding, efficiently created all of the combinations of parental properties, producing a great diversity of property combinations in the progeny enzymes. Thus, molecular… CONTINUE READING

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