DNA shuffling of a family of genes from diverse species accelerates directed evolution

@article{Crameri1998DNASO,
  title={DNA shuffling of a family of genes from diverse species accelerates directed evolution},
  author={Andreas Crameri and Sun Ai Raillard and Ericka Bermudez and Willem Pim C Stemmer},
  journal={Nature},
  year={1998},
  volume={391},
  pages={288-291}
}
DNA shuffling is a powerful process for directed evolution, which generates diversity by recombination,, combining useful mutations from individual genes. Libraries of chimaeric genes can be generated by random fragmentation of a pool of related genes, followed by reassembly of the fragments in a self-priming polymerase reaction. Template switching causes crossovers in areas of sequence homology. Our previous studies used single genes and random point mutations as the source of diversity. An… Expand
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