DNA sequencing with direct blotting electrophoresis and colorimetric detection.

@article{Richterich1989DNASW,
  title={DNA sequencing with direct blotting electrophoresis and colorimetric detection.},
  author={P Richterich and Christian Heller and H Wurst and Fritz M. Pohl},
  journal={BioTechniques},
  year={1989},
  volume={7 1},
  pages={52-9}
}
We describe optimized procedures for colorimetrically-detected DNA sequencing with direct blotting electrophoresis. One-step protocols for Sequenase and Klenow enzyme are given. The clapping technique has been adapted to allow convenient casting of very thin gels with an optimal lower gel (transfer) surface. This gives very sharp band patterns, enabling more than 350 bases from a single loading to be read with confidence. The crucial points for direct blotting electrophoresis are discussed… CONTINUE READING