DNA sequences required for regulated expression of β-globin genes in murine erythroleukemia cells

  title={DNA sequences required for regulated expression of $\beta$-globin genes in murine erythroleukemia cells},
  author={Stephanie Wright and Amon Rosenthal and Richard A. Flavell and Frank G. Grosveld},

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A directly repeated sequence in the beta-globin promoter regulates transcription in murine erythroleukemia cells
The increase in beta DRE activity upon MEL cell differentiation and the more pronounced effects of mutations in both repeats of thebeta DRE have implications for the mechanism of action of the element in regulating beta-globin transcription and for mutational studies of other repetitive or redundant transcription elements.
DNA sequences involved in transcriptional regulation of the mouse beta-globin promoter in murine erythroleukemia cells
A fragment of the promoter region, from -106 to +26 relative to the RNA cap site, was found to be sufficient for regulated transcription in MEL cells following induction of differentiation by dimethyl sulfoxide.
Expression of the human gamma-globin gene after retroviral transfer to transformed erythroid cells.
Regulation of the human fetal (gamma) globin gene and a series of mutant gamma-globin genes was studied after retroviral transfer into erythroid cells with fetal or adult patterns of endogenous
Specific expression of a foreign β-globin gene in erythroid cells of transgenic mice
These studies demonstrate that regulatory sequences closely linked to the β-globin gene are sufficient to specify a correct pattern of tissue-specific expression in a developing mouse, when the gene is integrated at a subset of foreign chromosomal positions.
Upstream G gamma-globin and downstream beta-globin sequences required for stage-specific expression in transgenic mice
Positive regulatory elements upstream from the G gamma-globin and downstream from the beta- globin gene are involved in the differential expression of the two genes during development.
Expression of human beta-globin genes in transgenic mice: effects of a flanking metallothionein-human growth hormone fusion gene
The results suggest that sequences which control metallothionein-human growth hormone gene expression are capable of stimulating the expression of a flanking gene in an orientation-independent and tissue-specific manner.
Activation of the Human jl-Globin Promoter in K 562 Cells by DNA Sequences 5 ' to the Fetal Ay-or Embryonic t-Globin Genes
Regulatory sequences of the human fetal -t-globin gene were studied by constructing composite 'y/ft globin promoters and comparing their function to that of intact , promoters in human K562 cells, which showed that the gene appeared to be inducible with hemin.
Erythroleukemia Cells and in Transgenic Mice Comparison of Expression of Human Globin Genes Transferred Into Mouse
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Regulated expression of the human β-globin gene family in murine erythroleukaemia cells
It is shown here that expression of the β, but not the γ or ε genes, is regulated during MEL differentiation.
DNA sequences necessary for transcription of the rabbit β-globin gene in vivo
Comparison of the level of β-globin transcripts in a variety of deletion mutants shows that for efficient transcription, both the ATA or Goldberg–Hogness box, and a region between 100 and 58 base pairs in front of the site at which transcription is initiated, are required.
The 5'-flanking region of a human IFN-alpha gene mediates viral induction of transcription.
It is concluded that induction acts by activating transcription rather than by reducing turnover, and that the regulatory elements are contained in the 5'-flanking region of the interferon gene.
Sequence requirements for the transcription of the rabbit beta-globin gene in vivo: the -80 region.
Analysis of DNA sequences in the -80 region of the rabbit beta-globin gene shows that the conserved GGCCAATCT sequence is required for efficient transcription in vivo and further identifies another sequence in the region from about -81 to -96 which is also required for transcription in vitro.
Transfer of human globin genes to erythroleukemic mouse cells.
Thymidine kinase negative (TK‐) Friend cells were transformed with recombinant molecules carrying human globin genes and the thymidine kinase gene of herpes simplex virus type 1 DNA. Transformation