DNA sequence and functions of the actVI region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor A3(2).

@article{FernndezMoreno1994DNASA,
  title={DNA sequence and functions of the actVI region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor A3(2).},
  author={Miguel {\'A}ngel Fern{\'a}ndez-Moreno and Eriel Mart{\'i}nez and J Caballero and Koji Ichinose and David A. Hopwood and Francisco Malpartida},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 40},
  pages={24854-63}
}
Six open reading frames (ORFs) were identified by DNA sequencing of 5.7 kilobase pairs at the left end of the act cluster (the so-called "actVI region"), in the order: ORFB, ORFA, ORF1, ORF2, ORF3, ORF4. ORF1-4 are transcribed rightward and in the same direction as the ORFs of the actVA region which lies to the right of the actVI region, whereas ORFA and ORFB run in the opposite direction. By complementation of mutants and gene disruption of the wild type strain, the two previously genetically… CONTINUE READING

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Mentioned Connections BETA
MutationNo subtypemutant
By complementation of mutants and gene disruption of the wild type strain , the two previously genetically characterized actVI mutations were assigned to ORF1 .
OxidoreductaseIs biochemical function of gene product3-Hydroxyacyl CoA Dehydrogenases
The ORF1 product strongly resembles beta - hydroxyacyl - CoA dehydrogenases of bacteria and mammals and the ORF2 and ORF4 products resemble each other and enoyl reductases from bacteria , animals , and plants , with a highly conserved cofactor - binding domain .
The ORF1 product strongly resembles beta - hydroxyacyl - CoA dehydrogenases of bacteria and mammals and the ORF2 and ORF4 products resemble each other and enoyl reductases from bacteria , animals , and plants , with a highly conserved cofactor - binding domain .
The ORF1 product strongly resembles beta - hydroxyacyl - CoA dehydrogenases of bacteria and mammals and the ORF2 and ORF4 products resemble each other and enoyl reductases from bacteria , animals , and plants , with a highly conserved cofactor - binding domain .
The ORF1 product strongly resembles beta - hydroxyacyl - CoA dehydrogenases of bacteria and mammals and the ORF2 and ORF4 products resemble each other and enoyl reductases from bacteria , animals , and plants , with a highly conserved cofactor - binding domain .
RNAChemical or drug is product of biological processTranscription, Genetic
RNA analysis within the ORF1/ORFA intergenic region showed overlapping divergent promoters , at least one of which is under the control of the actII - ORF4 gene product , the transcriptional activator of the act cluster .
Six open reading frames ( ORFs ) were identified by DNA sequencing of 5.7 kilobase pairs at the left end of the act cluster ( the so - called " actVI region " ) , in the order : ORFB , ORFA , ORF1 , ORF2 , ORF3 , ORF4 .
Six open reading frames ( ORFs ) were identified by DNA sequencing of 5.7 kilobase pairs at the left end of the act cluster ( the so - called " actVI region " ) , in the order : ORFB , ORFA , ORF1 , ORF2 , ORF3 , ORF4 .
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