DNA restriction-modification systems in the ethanologen, Zymomonas mobilis ZM4

  title={DNA restriction-modification systems in the ethanologen, Zymomonas mobilis ZM4},
  author={Aidan L. Kerr and Young Jae Jeon and Charles J. Svenson and Peter L. Rogers and Brett A Neilan},
  journal={Applied Microbiology and Biotechnology},
To better understand the DNA restriction-modification (R-M) systems for more amenable strain development of the alternative industrial ethanologen, Zymomonas mobilis, three gene knockout mutants were constructed. The gene knockout mutants were tested for their DNA restriction activities by the determination of transformation efficiency using methylated and unmethylated foreign plasmid DNAs. Inactivation of a putative mrr gene encoded by ZMO0028 (zmrr) resulted in a 60-fold increase in the… CONTINUE READING
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