DNA reshaping by MukB. Right-handed knotting, left-handed supercoiling.

  title={DNA reshaping by MukB. Right-handed knotting, left-handed supercoiling.},
  author={Zoya M Petrushenko and Chien-Hung Lai and Rachna Rai and Valentin V Rybenkov},
  journal={The Journal of biological chemistry},
  volume={281 8},
MukB is a bacterial SMC (structural maintenance of chromosome) protein required for faithful chromosome segregation in Escherichia coli. We report here that purified MukB introduces right-handed knots into DNA in the presence of type-2 topoisomerase, indicating that the protein promotes intramolecular DNA condensation. The pattern of generated knots suggests that MukB, similar to eukaryotic condensins, stabilizes large right-handed DNA loops. In contrast to eukaryotic condensins, however, the… CONTINUE READING

From This Paper

Topics from this paper.


Publications citing this paper.
Showing 1-10 of 32 extracted citations

Biochemical Analysis of Bacterial Condensins.

Methods in molecular biology • 2017
View 10 Excerpts

MukBEF, a chromosomal organizer.

Journal of molecular microbiology and biotechnology • 2014

Noncanonical SMC protein in Mycobacterium smegmatis restricts maintenance of Mycobacterium fortuitum plasmids.

Proceedings of the National Academy of Sciences of the United States of America • 2014
View 1 Excerpt

Similar Papers

Loading similar papers…