DNA reshaping by MukB. Right-handed knotting, left-handed supercoiling.

@article{Petrushenko2006DNARB,
  title={DNA reshaping by MukB. Right-handed knotting, left-handed supercoiling.},
  author={Zoya M Petrushenko and Chien-Hung Lai and Rachna Rai and Valentin V Rybenkov},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 8},
  pages={4606-15}
}
MukB is a bacterial SMC (structural maintenance of chromosome) protein required for faithful chromosome segregation in Escherichia coli. We report here that purified MukB introduces right-handed knots into DNA in the presence of type-2 topoisomerase, indicating that the protein promotes intramolecular DNA condensation. The pattern of generated knots suggests that MukB, similar to eukaryotic condensins, stabilizes large right-handed DNA loops. In contrast to eukaryotic condensins, however, the… CONTINUE READING

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