DNA replication in cell-free extracts from Drosophila melanogaster.

@article{Crevel1991DNARI,
  title={DNA replication in cell-free extracts from Drosophila melanogaster.},
  author={Gilles Crevel and Sue Cotterill},
  journal={The EMBO journal},
  year={1991},
  volume={10 13},
  pages={
          4361-9
        }
}
We have developed an efficient in vitro replication system from 0-2 h Drosophila melanogaster embryos. Demembranated Xenopus sperm DNA when incubated in such an extract first becomes enclosed in a nucleus-like structure with a nuclear envelope and a karyoskeleton. It then undergoes one round of semiconservative replication--this replication appears completely dependent on nuclear formation. Up to 30% of input DNA is nucleated in one reaction. Efficient nuclear formation and replication are… CONTINUE READING
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