DNA replication in cell-free extracts from Drosophila melanogaster.

  title={DNA replication in cell-free extracts from Drosophila melanogaster.},
  author={Gilles Crevel and Sue Cotterill},
  journal={The EMBO journal},
  volume={10 13},
We have developed an efficient in vitro replication system from 0-2 h Drosophila melanogaster embryos. Demembranated Xenopus sperm DNA when incubated in such an extract first becomes enclosed in a nucleus-like structure with a nuclear envelope and a karyoskeleton. It then undergoes one round of semiconservative replication--this replication appears completely dependent on nuclear formation. Up to 30% of input DNA is nucleated in one reaction. Efficient nuclear formation and replication are… CONTINUE READING
Highly Cited
This paper has 19 citations. REVIEW CITATIONS

From This Paper

Topics from this paper.


Publications citing this paper.
Showing 1-10 of 10 citations