DNA replication errors produced by the replicative apparatus of Escherichia coli.

@article{Fujii1999DNARE,
  title={DNA replication errors produced by the replicative apparatus of Escherichia coli.},
  author={S Fujii and Masahiro Tatsumi Akiyama and Ken-ichi Aoki and Yorihito Sugaya and Kumiko Higuchi and Miki Hiraoka and Yuko Miki and Nakamichi Saitoh and Kaoru Yoshiyama and Kenji Ihara and Motoaki Seki and Eiichi Ohtsubo and Hisaji Maki},
  journal={Journal of molecular biology},
  year={1999},
  volume={289 4},
  pages={
          835-50
        }
}
It has been hard to detect forward mutations generated during DNA synthesis in vitro by replicative DNA polymerases, because of their extremely high fidelity and a high background level of pre-existing mutations in the single-stranded template DNA used. Using the oriC plasmid DNA replication in vitro system and the rpsL forward mutation assay, we examined the fidelity of DNA replication catalyzed by the replicative apparatus of Escherichia coli. Upon DNA synthesis by the fully reconstituted… CONTINUE READING

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