DNA replication-coupled PCNA mono-ubiquitination and polymerase switching in a human in vitro system.

@article{Masuda2010DNARP,
  title={DNA replication-coupled PCNA mono-ubiquitination and polymerase switching in a human in vitro system.},
  author={Yuji Masuda and Jinlian Piao and Kenji Kamiya},
  journal={Journal of molecular biology},
  year={2010},
  volume={396 3},
  pages={487-500}
}
Translesion DNA synthesis is a mechanism of DNA damage tolerance, and mono-ubiquitination of proliferating cell nuclear antigen (PCNA) is considered to play a key role in regulating the switch from replicative to translesion DNA polymerases (pols). In this study, we analyzed effects of a replicative pol delta on PCNA mono-ubiquitination with the ubiquitin-conjugating enzyme and ligase UBE2A/HHR6A/RAD6A-RAD18. The results revealed that PCNA interacting with pol delta is a better target for… CONTINUE READING