Replication protein A: directing traffic at the intersection of replication and repair.
- Biology, ChemistryFrontiers in bioscience
This review summarizes the current understanding of RPA structure, phosphorylation and protein-protein interactions in mediating these DNA metabolic processes.
Dna2 on the road to Okazaki fragment processing and genome stability in eukaryotes
- BiologyCritical reviews in biochemistry and molecular biology
The function of Dna2 in Okazaki fragment processing is described, its role in the maintenance of genome integrity with an emphasis on its functional interactions with other factors required for genome maintenance is discussed, and many roles of Dn2 suggest that the preemptive removal of long or structured flaps ultimately contributes to genome maintenance in eukaryotes.
Okazaki fragment maturation: nucleases take centre stage.
- BiologyJournal of molecular cell biology
The dynamic interactions of polymerase δ, FEN1 and DNA ligase I with proliferating cell nuclear antigen allow these enzymes to act sequentially during Okazaki fragment maturation and the distinct roles of these nucleases in different pathways for removal of RNA/DNA primers are summarized.
Running into problems: how cells cope with replicating damaged DNA.
- BiologyMutation research
Characterizing the Final Steps of Chromosomal Replication at the Single-molecule Level in the Model System Escherichia coli
Characterizing the Final Steps of Chromosomal Replication at the Single-molecule Level in the Model System Escherichia coli shows good consistency with previous work on this topic.
Dynamics of replication proteins during lagging strand synthesis: A crossroads for genomic instability and cancer.
- Biology, ChemistryDNA repair
Identification of short ‘eukaryotic’ Okazaki fragments synthesized from a prokaryotic replication origin
- BiologyEMBO reports
Mapping of replication initiation points showed that discontinuous DNA replication in P. abyssi starts at a well‐defined site within the oriC recently identified in this hyperthermophile, which implies a very efficient turnover of Okazaki fragments in Archaea.
The diverse spectrum of sliding clamp interacting proteins
- BiologyFEBS letters
The structure and function of replication protein A in DNA replication.
- BiologySub-cellular biochemistry
This chapter summarizes the current understanding of RPA's roles in replication by reviewing the available structural data, DNA-binding properties, interactions with various replication proteins, and interactions with DNA repair proteins when DNA replication is stalled.
Lagging Strand Synthesis and Genomic Stability
In eukaryotic cells, DNA replication starts at many origins in each chromosome during S phase of cell cycle, where activation of origins leads to the establishment of bidirectional replication forks for the DNA synthesis of leading and lagging strands.
SHOWING 1-10 OF 16 REFERENCES
The DNA replication fork in eukaryotic cells.
- BiologyAnnual review of biochemistry
Biochemical studies, principally of plasmid DNAs containing the Simian Virus 40 origin of DNA replication, and yeast genetic studies have uncovered the fundamental mechanisms of replication fork progression.
RPA governs endonuclease switching during processing of Okazaki fragments in eukaryotes
It is shown that the endonucleases Dna2 and Fen1 act sequentially to facilitate the complete removal of the primer RNA in Okazaki fragments, governed by a single-stranded DNA-binding protein, replication protein-A (RPA).
Characterization of the Enzymatic Properties of the Yeast Dna2 Helicase/Endonuclease Suggests a New Model for Okazaki Fragment Processing*
- Biology, ChemistryThe Journal of Biological Chemistry
A new model in which Dna2 plays a direct role in Okazaki fragment maturation in conjunction with Fen-1 is proposed, in which it is demonstrated that the removal of pre-existing initiator 5′-terminal RNA segments depended on a displacement reaction carried out during the DNA polymerase δ-catalyzed elongation of the upstream Okazaki fragments.
Saccharomyces cerevisiae RNase H(35) Functions in RNA Primer Removal during Lagging-Strand DNA Synthesis, Most Efficiently in Cooperation with Rad27 Nuclease
- BiologyMolecular and Cellular Biology
The substrate specificities and the cooperative as well as independent cleavage mechanisms of Saccharomyces cerevisiae RNase H(35) and Rad27 nuclease are defined by using Okazaki fragment model substrates and the additive and compensatory pathological effects of gene deletion and overexpression of these two enzymes are determined.
A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function
- BiologyMolecular and cellular biology
It is shown that the yeast F EN-1 (yFEN-1) nuclease interacts genetically and biochemically with Dna2 helicase, a new helicase that is essential for yeast DNA replication and repair.
DNA2 Encodes a DNA Helicase Essential for Replication of Eukaryotic Chromosomes (*)
- BiologyThe Journal of Biological Chemistry
It is shown that the helicase domain is required in vivo and that a 3′ to 5′ DNA helicase activity specific for forked substrates is intrinsic to the Dna2p, which is essential for DNA replication.
A yeast gene required for DNA replication encodes a protein with homology to DNA helicases.
- BiologyProceedings of the National Academy of Sciences of the United States of America
A yeast gene has been identified by screening for DNA replication mutants using a permeabilized cell replication assay and the gene encodes a 172-kDa protein with characteristic DNA helicase motifs.
The impact of lagging strand replication mutations on the stability of CAG repeat tracts in yeast.
The stability of long tracts of CAG repeats in yeast mutants defective in enzymes suspected to be involved in lagging strand replication is examined in terms of the steps of replication that are likely to lead to expansion and to contraction of C AG repeat tracts.
The endonuclease activity of the yeast Dna2 enzyme is essential in vivo.
- Biology, ChemistryNucleic acids research
In vivo and in vitro results demonstrate that the endonuclease activity of Dna2 is essential for Okazaki fragment processing.
The Nuclease Activity of the Yeast Dna2 Protein, Which Is Related to the RecB-like Nucleases, Is Essential in Vivo *
- Biology, ChemistryThe Journal of Biological Chemistry
Physiological analysis shows that both ATPase and nuclease are important for the essential function of DNA2 in DNA replication and for its role in double-strand break repair.