DNA repair modifies the site and strand specificity of ethyl methanesulfonate mutagenesis in yeast.

@article{Kunz1992DNARM,
  title={DNA repair modifies the site and strand specificity of ethyl methanesulfonate mutagenesis in yeast.},
  author={Bernard A. Kunz and Mbalaviele Gabriel and Xiaolin Kang and Susanne E Kohalmi and Karin A. Terrick},
  journal={Mutagenesis},
  year={1992},
  volume={7 6},
  pages={
          461-9
        }
}
The influence of DNA repair on the specificity of ethyl methanesulfonate (EMS) mutagenesis in a plasmid-borne copy of the Saccharomyces cerevisiae SUP4-o gene was investigated. Isogenic yeast strains that are repair-proficient (RAD) or defective for nucleotide excision (rad1), postreplication (rad18) or recombinational repair (rad52) were treated with EMS. Compared to the RAD wild-type, the maximum SUP4-o mutation frequency was 2-fold greater in the rad1 background whereas it was approximately… CONTINUE READING