DNA rearrangements associated with a transposable element in yeast.

  title={DNA rearrangements associated with a transposable element in yeast.},
  author={G. Shirleen Roeder and Gerald Fink},
  volume={21 1},
The his4-912 mutation results from insertion of a 6200 bp transposable element into the his4 gene of yeast. In order to clone the his4-912 mutation, the plasmid pBR322 was integrated into the his4 gene by means of yeast transformation, and then the vector sequences and the his4-912 insertion element were excised as a single restriction fragment. This his 4-912 insertion element is homologous to Ty1, a family of repetitive yeast DNA sequences. His+ revertants derived from the his4-912 mutant… CONTINUE READING


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