DNA-protein interactions at the S.cerevisiae alpha 2 operator in vivo.

@article{Murphy1993DNAproteinIA,
  title={DNA-protein interactions at the S.cerevisiae alpha 2 operator in vivo.},
  author={Michael R. Murphy and Makoto Shimizu and Sharon Y. Roth and Anne M. Dranginis and R. T. Simpson},
  journal={Nucleic acids research},
  year={1993},
  volume={21 14},
  pages={3295-300}
}
Two homodimeric proteins, alpha 2 and MCM1, are required to repress transcription of a-cell type specific genes in haploid yeast alpha-cells. In vitro studies by others of the interactions of these proteins with operator DNA have suggested that MCM1 binds to the middle and alpha 2 to the ends of the 31 bp operator. We have previously shown that alpha 2 organizes chromatin structure adjacent to the operator; in the presence of alpha 2 repressor, a precisely positioned nucleosome abuts the… CONTINUE READING