• Corpus ID: 13287260

DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.

@article{Folmer1994DNAPF,
  title={DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.},
  author={O. F. Folmer and Michael B. Black and Walter Randolph Hoeh and R. V. Lutz and Robert C. Vrijenhoek},
  journal={Molecular marine biology and biotechnology},
  year={1994},
  volume={3 5},
  pages={
          294-9
        }
}
We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. [] Key Result Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at…
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References

SHOWING 1-10 OF 16 REFERENCES

Nucleotide sequence and gene organization of sea urchin mitochondrial DNA.

The CO-I and CO-II region of honeybee mitochondrial DNA: evidence for variation in insect mitochondrial evolutionary rates.

Parsimony analyses using the mouse and Xenopus sequences as outgroups show significantly more amino acid substitutions on the branch to Apis than on that to Drosophila, indicating a difference in the long-term evolutionary rates of hymenopteran and dipteran mtDNA.

Sequence and organization of the human mitochondrial genome

The complete sequence of the 16,569-base pair human mitochondrial genome is presented and shows extreme economy in that the genes have none or only a few noncoding bases between them, and in many cases the termination codons are not coded in the DNA but are created post-transcriptionally by polyadenylation of the mRNAs.

The mitochondrial genome of the mosquito Anopheles gambiae: DNA sequence, genome organization, and comparisons with mitochondrial sequences of other insects

The entire 15,363 bp mitochondrial genome was cloned and sequenced from the mosquito Anopheles gambiae and compared with the mitochondrial genomes of other insects on the basis genome size and organization, DNA and putative amino acid sequence data, nucleotide substitutions, codon usage and bias, and patterns of AT enrichment.

Sequence and gene organization of mouse mitochondrial DNA

Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome.

Sequence and gene organization of the chicken mitochondrial genome. A novel gene order in higher vertebrates.

Evidence for the frequent use of TTG as the translation initiation codon of mitochondrial protein genes in the nematodes, Ascaris suum and Caenorhabditis elegans.

Data obtained from alignments of nucleotide sequences of mitochondrial (mt) DNA molecules of the nematode worms Ascaris suum and Caenorhabditis elegans indicate that, as found in other metazoan mitochondria, 3'-terminal polyadenylation is occasionally necessary to generate complete translation termination codons in transcripts of nematodes mt-protein genes.

The complete nucleotide sequence of the Xenopus laevis mitochondrial genome.

On the Vestimentifera, new phylum: six new species, and other taxa, from hydrothermal vents and elsewhere

Le sousphylum obturata (phylum des Pogonophora) au rang d'un nouveau phylum les Vestimentifera est restaure dans son statut original comme phylum Pogonphora.