• Corpus ID: 13287260

DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.

  title={DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.},
  author={O. F. Folmer and Michael B. Black and Walter Randolph Hoeh and R. V. Lutz and Robert C. Vrijenhoek},
  journal={Molecular marine biology and biotechnology},
  volume={3 5},
We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. [] Key Result Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at…

Figures and Tables from this paper

Redesign of PCR primers for mitochondrial cytochrome c oxidase subunit I for marine invertebrates and application in all‐taxa biotic surveys

The new primers, jgHCO2198 and jgLCO1490, are well suited for routine DNA barcoding, all‐taxon surveys and metazoan metagenomics.

A New Primer Set to Amplify the Mitochondrial Cytochrome C Oxidase Subunit I (COI) Gene in the DHA-Rich Microalgae, the Genus Aurantiochytrium

A primer set for mitochondrial cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium amplified sequences from Aurant Antiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiacytrium in the field.

A set of new primers for COI (cytochrome oxidase subunit 1 mitochondrial gene) amplification in Phyllodiaptomus tunguidus (Copepoda, Calanoida, Diaptomidae)

A new pair of primers are developed for Phyllodiaptomus tunguidus with significantly higher amplification success rate, which will facilitate the recovery of barcode data from this and probably other copepod species, and stimulate further studies of phylogeny and genetic diversity on P. tungUIDus.

New primers for amplification of cytochrome c oxidase subunit I barcode region designed for species of Decapoda (Crustacea)

14 new primers for amplification of the COI barcode region of decapod crustacean species are designed and tested, with high level of success, and the applicability of these primers is expected for several studies with different taxa within Decapoda.

Universal cytochrome b primers facilitate intraspecific studies in molluscan taxa.

The construction of amplification primers designed to target a portion of the mitochondrial cytochrome b locus in a variety of molluscan taxa are described and the utility of these primer sets for studies of intraspecific phylogeny inmollusks and potentially other invertebrates is discussed.

Sequencing of Cytochrome C Oxidase Subunit I Gene of Mitochondrial DNA from Chelonia mydas in Qatar.

DNA of Qatari C. mydas samples were successfully sequenced using the Folmer forward and reverse primers and revealed maximum homology with C.mydas, which is a species of turtle declared "extinct in wild" by IUCN.

Phylogeny of leeches (Hirudinea) based on mitochondrial cytochrome c oxidase subunit I.

Modern taxonomic groupings of leeches into higher categories were found to be largely consistent with monophyletic groups identified in the analysis, including a sister-group relationship between the piscicolids and Arhynchobdellida.

Mitochondrial DNA phylogeny of red and green rock lobsters (genus Jasus)

A cladistic analysis of nucleotide substitutions in the 16S ribosomal RNA and cytochrome oxidase subunit I genes of mitochondrial DNA does not support the monophyly of species within each of the

Chon Buri Province Using Partial Sequences of Mitochondrial 16 S rRNA , 12 S rRNA and Cytochrome c Oxidase Subunit I ( COI ) Genes อเด ยามินดาโก

This work verified Morphological Identification of Grapsid Crabs Genus Metopograpsus H. Milne Edwards, 1853 from Chon Buri Province using Partial Sequences of Mitochondrial 16S rRNA, 12S r RNA and Cytochrome c Oxidase Subunit I (COI) Genes.

Recovery of the mitochondrial COI barcode region in diverse Hexapoda through tRNA-based primers

Results show that primers designed to bind to highly conserved gene regions upstream of COI will aid the amplification of this gene region in species where standard primers fail and provide valuable information to design a primer for problem groups.



The CO-I and CO-II region of honeybee mitochondrial DNA: evidence for variation in insect mitochondrial evolutionary rates.

Parsimony analyses using the mouse and Xenopus sequences as outgroups show significantly more amino acid substitutions on the branch to Apis than on that to Drosophila, indicating a difference in the long-term evolutionary rates of hymenopteran and dipteran mtDNA.

Sequence and organization of the human mitochondrial genome

The complete sequence of the 16,569-base pair human mitochondrial genome is presented and shows extreme economy in that the genes have none or only a few noncoding bases between them, and in many cases the termination codons are not coded in the DNA but are created post-transcriptionally by polyadenylation of the mRNAs.

The mitochondrial genome of the mosquito Anopheles gambiae: DNA sequence, genome organization, and comparisons with mitochondrial sequences of other insects

The entire 15,363 bp mitochondrial genome was cloned and sequenced from the mosquito Anopheles gambiae and compared with the mitochondrial genomes of other insects on the basis genome size and organization, DNA and putative amino acid sequence data, nucleotide substitutions, codon usage and bias, and patterns of AT enrichment.

Evidence for the frequent use of TTG as the translation initiation codon of mitochondrial protein genes in the nematodes, Ascaris suum and Caenorhabditis elegans.

Data obtained from alignments of nucleotide sequences of mitochondrial (mt) DNA molecules of the nematode worms Ascaris suum and Caenorhabditis elegans indicate that, as found in other metazoan mitochondria, 3'-terminal polyadenylation is occasionally necessary to generate complete translation termination codons in transcripts of nematodes mt-protein genes.

On the Vestimentifera, new phylum: six new species, and other taxa, from hydrothermal vents and elsewhere

Le sousphylum obturata (phylum des Pogonophora) au rang d'un nouveau phylum les Vestimentifera est restaure dans son statut original comme phylum Pogonphora.