DNA polymerases eta and theta function in the same genetic pathway to generate mutations at A/T during somatic hypermutation of Ig genes.

@article{Masuda2007DNAPE,
  title={DNA polymerases eta and theta function in the same genetic pathway to generate mutations at A/T during somatic hypermutation of Ig genes.},
  author={Keiji Masuda and Rika Ouchida and Masaki Hikida and Tomohiro Kurosaki and Masayuki Yokoi and Chikahide Masutani and Mineaki Seki and Richard D Wood and Fumio Hanaoka and Jiyang O-Wang},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 24},
  pages={17387-94}
}
Somatic hypermutation of the Ig genes requires the activity of multiple DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs. Mice deficient for DNA polymerase eta (POLH) exhibited an approximately 80% reduction of the mutations at A/T, whereas absence of polymerase (POLQ) resulted in approximately 20% reduction of both A/T and C/G mutations. To investigate whether the residual A/T mutations observed in the absence of POLH are generated by POLQ and how these two… CONTINUE READING