DNA polymerase profiling.

Abstract

We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template strand. A resulting distance alteration is reported by fluorescence resonance energy transfer between two dyes… (More)
DOI: 10.1007/978-1-60327-040-3_16

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Cite this paper

@article{Summerer2008DNAPP, title={DNA polymerase profiling.}, author={Daniel Summerer}, journal={Methods in molecular biology}, year={2008}, volume={429}, pages={225-35} }