DNA polymerase-catalyzed addition of nontemplated extra nucleotides to the 3' end of a DNA fragment.

@article{Hu1993DNAPA,
  title={DNA polymerase-catalyzed addition of nontemplated extra nucleotides to the 3' end of a DNA fragment.},
  author={Gengxi Hu},
  journal={DNA and cell biology},
  year={1993},
  volume={12 8},
  pages={763-70}
}
  • Gengxi Hu
  • Published 1993 in DNA and cell biology
Some prokaryotic and eukaryotic DNA polymerases are capable of adding an additional nontemplated nucleotide residue at the 3' end of a DNA fragment (Clark et al., 1987; Clark, 1988). The extra nucleotide at the 3' end of the PCR product has been shown to be a critical factor determining the efficiency of cloning PCR products into plasmids and can affect mutation analyses with a PCR-denaturing gradient gel electrophoresis (DGGE) approach (Pfeiffer and Hu, 1993). In the present work, the ability… CONTINUE READING

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