DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair.

@article{Liu2005DNAPB,
  title={DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair.},
  author={Yuan Liu and William A. Beard and David D. Shock and Rajendra Prasad and Esther W. Hou and Samuel H. Wilson},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 5},
  pages={3665-74}
}
DNA polymerase beta (pol beta) and flap endonuclease 1 (FEN1) are key players in pol beta-mediated long-patch base excision repair (LP-BER). It was proposed that this type of LP-BER is accomplished through FEN1 removal of a 2- to 11-nucleotide flap created by pol beta strand displacement DNA synthesis. To understand how these enzymes might cooperate during LP-BER, we characterized purified human pol beta DNA synthesis by utilizing various BER intermediates, including single-nucleotide-gapped… CONTINUE READING