DNA polymerase beta and PARP activities in base excision repair in living cells.

@article{Masaoka2009DNAPB,
  title={DNA polymerase beta and PARP activities in base excision repair in living cells.},
  author={Aya Masaoka and Julie K. Horton and William A. Beard and Samuel H. Wilson},
  journal={DNA repair},
  year={2009},
  volume={8 11},
  pages={1290-9}
}
To examine base excision repair (BER) capacity in the context of living cells, we developed and applied a plasmid-based reporter assay. Non-replicating plasmids containing unique DNA base lesions were designed to express luciferase only after lesion repair had occurred, and luciferase expression in transfected cells was measured continuously during a repair period of 14 h. Two types of DNA lesions were examined: uracil opposite T reflecting repair primarily by the single-nucleotide BER sub… CONTINUE READING

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