DNA polymerase II as a fidelity factor in chromosomal DNA synthesis in Escherichia coli

@article{BanachOrowska2005DNAPI,
  title={DNA polymerase II as a fidelity factor in chromosomal DNA synthesis in Escherichia coli},
  author={Magdalena Banach-Orłowska and Iwona J Fijalkowska and Roel M. Schaaper and Piotr Jonczyk},
  journal={Molecular Microbiology},
  year={2005},
  volume={58}
}
Escherichia coli DNA polymerase III holoenzyme (HE) is the main replicase responsible for replication of the bacterial chromosome. E. coli contains four additional polymerases, and it is a relevant question whether these might also contribute to chromosomal replication and its fidelity. Here, we have investigated the role of DNA polymerase II (Pol II) (polB gene product). Mismatch repair‐defective strains containing the polBex1 allele – encoding a polymerase‐proficient but exonucleolytically… 
Role of Escherichia coli DNA polymerase I in chromosomal DNA replication fidelity
TLDR
The data suggest that the additional errors produced by polAexo are created during the maturation of Okazaki fragments in the lagging strand, which is best explained by an effect that is additive compared with other error‐producing events at the replication fork.
Replication fidelity in E. coli: Differential leading and lagging strand effects for dnaE antimutator alleles.
TLDR
The present work used three different dnaE alleles that had previously been isolated as antimutators to gain deeper insight into the factors that influence the fidelity of chromosomal DNA replication in E. coli, and proposed that these dna E alleles are promising tools for studying polymerase exchanges at the replication fork.
Role of Accessory DNA Polymerases in DNA Replication in Escherichia coli: Analysis of the dnaX36 Mutator Mutant
TLDR
The present results support the assumption that dnaX36-mediated mutations originate as errors of replication rather than DNA damage-related events, and provide insight into the interplay of the various DNA polymerases, and of tau subunit, in securing a high fidelity of replication.
DNA polymerase switching: effects on spontaneous mutagenesis in Escherichia coli
TLDR
The observations suggest that there is considerable interplay among all five E. coli polymerases that either reduces or enhances the mutagenic load on the E.″coli chromosome.
DNA replication fidelity in Escherichia coli: a multi-DNA polymerase affair.
TLDR
This review is concerned with the mechanisms underlying the fidelity of the chromosomal replication in the model system Escherichia coli by DNA polymerase III holoenzyme, with further emphasis on participation of the other, accessory DNA polymerases, of which E. coli contains four (Pols I, II, IV, and V).
Interplay between replication and recombination in Escherichia coli: impact of the alternative DNA polymerases.
  • Stéphane Delmas, I. Matic
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 2006
TLDR
The results suggest that there is probably not an active choice of which pathway to use, but, rather, the nature and concentration of lesions that lead to formation of ssDNA and the level of SOS induction that they engender might determine the outcome of the competition between HR and alternative DNA polymerases.
Interactions of the Bacillus subtilis DnaE polymerase with replisomal proteins modulate its activity and fidelity
TLDR
In vivo data show that protein–protein interactions within the replisome modulate the activity and fidelity of DnaE, and confirm the prominent role of DnE during B. subtilis replication.
Competition of Escherichia coli DNA Polymerases I, II and III with DNA Pol IV in Stressed Cells
TLDR
The data support the remaining hypothesis that during the acts of DNA double-strand-break (DSB) repair, shown previously to underlie stress-induced mutagenesis in the Lac system, there is competition of DNA polymerases I, II and III with DNA Pol IV for action at the primer terminus.
Elevated expression of DNA polymerase II increases spontaneous mutagenesis in Escherichia coli.
TLDR
It is shown that elevated expression of Pol-II leads to an approximately 100-fold increase in spontaneous mutagenesis in a manner that is independent of SOS, umuDC, dinB, recA, uvrA and mutS functions, and introduces a mutation in the proofreading exo motif of polB that elevatesmutagenesis by a further 180-fold, suggesting that Pol- II can effectively compete with DNA polymerase III for DNA synthesis.
Proofreading deficiency of Pol I increases the levels of spontaneous rpoB mutations in E. coli.
TLDR
Data indicated that in a strain carrying a proofreading-exonuclease-defective form of Pol I (polAexo mutant) the frequency of rpoB mutations increased by about 2-fold, consistent with a model that the fidelity of DNA polymerase I is important in controlling the overall fidelity of chromosomal DNA replication.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 76 REFERENCES
Escherichia coli DNA polymerase II catalyzes chromosomal and episomal DNA synthesis in vivo.
TLDR
Pol II exo- caused a significant increase in the frequency of base substitution and frameshift mutations on F' episomes, even in dnaE+ cells, suggesting that Pol II is able to compete with Pol III for DNA synthesis on F episomes.
The Escherichia coli polB Locus Is Identical to dinA, the Structural Gene for DNA Polymerase II
TLDR
The sequence data reveal that polB and dinA represent the same gene and that the original transduction mapping of polB was inaccurate, suggesting that the normal level of exonucleolytic proofreading associated with the mutant Pol B100 enzyme may explain the repeated failures, over the past two decades, to detect phenotypes in polB mutant strains.
Proofreading-defective DNA polymerase II increases adaptive mutation in Escherichia coli.
The role of Escherichia coli DNA polymerase (Pol) II in producing or avoiding mutations was investigated by replacing the chromosomal Pol II gene (polB+) by a gene encoding an exonuclease-deficient
Roles of chromosomal and episomal dinB genes encoding DNA pol IV in targeted and untargeted mutagenesis in Escherichia coli
TLDR
It is reported that DNA pol IV is involved in –1 frameshift mutagenesis induced by 4-nitroquinoline N-oxide (4-NQO) and that the expression level of the chromosomalPol IV gene is 6–12 times higher than those for other SOS-inducible DNA polymerases in E. coli.
Mutants of Escherichia coli with increased fidelity of DNA replication.
TLDR
Mutation rates were reduced in both mutL and mutT backgrounds, but mutagenesis by ultraviolet light was not significantly affected, suggesting that the antimutator effect may be largely restricted to normal DNA replication.
Role of Escherichia coli DNA Polymerase IV in In Vivo Replication Fidelity
TLDR
Virtually no contribution of Pol IV to the chromosomal mutation rate was observed, but a significant effect of dinB was observed for reversion of a lac allele when the lac gene resided on an F'(pro-lac) episome.
Fidelity and Error Specificity of the α Catalytic Subunit of Escherichia coli DNA Polymerase III*
TLDR
The results suggest that the α subunit has a relatively low ability to extend from misincorporated bases, accounting for the low level of observed base substitutions, and has a comparatively high capability of extension after misalignment of a misinc incorporated base on the next (complementary) template base, accounts for the high level of frameshift mutations.
A phenotype for enigmatic DNA polymerase II: a pivotal role for pol II in replication restart in UV-irradiated Escherichia coli.
TLDR
It is demonstrated that pol II and UmuD'(2)C act in independent pathways of replication restart, thereby providing a phenotype for pol II in the repair of UV-damaged DNA.
Unequal fidelity of leading strand and lagging strand DNA replication on the Escherichia coli chromosome.
TLDR
Analysis of the possible mispairs underlying each specific base pair substitution suggests that the lagging strand replication on the E. coli chromosome may be more accurate than leading strand replication, indicating that leading and lagged strand replication have differential fidelity.
Role of DNA polymerase II in repair replication in Escherichia coli.
TLDR
Empirical experiments show that the ultraviolet-stimulated nonconservative DNA synthesis observed in toluenized strains deficient in DNA polymerases I and III is absent in mutants additionally deficient inDNA polymerase II.
...
1
2
3
4
5
...